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Bright, photostable, and non-toxic for long-term live cell imaging. Lung Cell. Images are 3-D reconstructions created using the Daimeimaging program (see Daims et al., 2006, and Internet Resources) from stacks of optical sections collected with an LSM 510 . Rosamine dyes: orange, red and infra-red-fluorescent MitoTrackers ( 23 ). Keima-Red Priciple Mtphagy Dye (included in Mitophagy Detection Kit) is a pH-sensitive fluorescent probe that accumulates in mitochondria and emits red fluorescence due to acidic conditions in a lysosome. The nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI; blue fluorescence), while the mitochondria and actin cytoskeleton were stained with MitoTracker Red (red fluorescence) and a phalloidin derivative (green fluorescence), respectively. CMXROS is a red dye; hence, any co-staining will require a GFP-tagged protein or FITC-conjugated secondary antibody. Structure-specific small molecule dyes . Fluorescent Cell Stains for Organelles & Cellular Structures - Biotium MitoTracker Red CMXRos is a red-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. . Plate reader, microscope, flow cytometer. PDF Mitochondrial Membrane Potential/Annexin V Apoptosis Kit To make a 1 mM stock solution, reconstitute the solid in 94.1 μl high quality DMSO. a Flow cytometry analysis of MitoTracker Red uptake by MSCs (GFP+ gated) cocultured with MitoTracker Red-labeled Jurkat cells after 300 nM ara-C or 100 nM MTX was added for 48 h.b Flow cytometry analysis of MitoTracker Red uptake by Jurkat cells (GFP− gated) cocultured with MitoTracker Red-labeled GFP+ MSCs after 300 nM . TMRE-Mitochondrial Membrane Potential Assay Kit (ab113852) - Abcam Figure 1 - The Basic Principle of STORM Imaging. AEDG peptide . Part 1: Basic principles of lung stereology. When present in the same subcellular compartment, two fragments of GFP namely GFP β1-10 and GFP β11ch can auto-assemble to form a fluorescent BiG Mito-Split-GFP chaplet (three reconstituted GFPs). Please note: In principle, glass bottoms are suitable for direct cell culture. Washed out of mitochondria after fixation. Total acq. Principles of fluorescent probes - SlideServe PDF Mitotracker Deep Red Flow Cytometry Protocol Basic Concepts in Fluorescence - Florida State University To 1 mL of cells, add 4 μL of 10 μM MitoTracker® Red working solution from the above step and stain for 30 minutes at 37°C in an atmosphere of 5% CO 2. as MitoTracker® Red CMXRos*) is an red-fluorescent dye (Ex/Em (nm): 580/600) that stains mitochondria in live cells. Several spin traps with different principles of detection were used. simply incubated with MitoTracker® probes, which passively diffuse across the plasma membrane and accumulate in active mitochondria.